Journal: Journal of Materials Science

Article Title: Aligned carbon nanotube–epoxy composites: the effect of nanotube organization on strength, stiffness, and toughness

doi: 10.1007/s10853-016-0228-6

Figure Lengend Snippet: Figure 4 a CNTF-nanocomposite specimen, b and c X-ray tomography 3-D reconstruction, and d 2-D slice image of a representative CNTF-epoxy nanocomposite. Composites made from mats rather than CNT fibres show similar tomographic results.

Article Snippet: X-ray micro-tomography (Bruker SkyScan 1172) was used for microstructural characterization and three-dimensional (3-D) visualization (details in supplementary materials, S1).

Techniques: Tomography

Journal: Theranostics

Article Title: Desktop-stereolithography 3D printing of a radially oriented extracellular matrix/mesenchymal stem cell exosome bioink for osteochondral defect regeneration

doi: 10.7150/thno.31017

Figure Lengend Snippet: Attenuation of chondrocyte degeneration by MSC exosomes. (A) Morphology of exosomes imaged by TEM. (B) Western blotting of the exosome markers TSG101, CD9, and CD63. (C) Particle size distribution of exosomes. (D) Representative immunofluorescence micrograph of DiO (green)-labeled exosomes taken up by chondrocytes. Scale bar = 100 μm. (E) Confocal microscopy of chondrocytes incubated with DiO-labeled exosomes (green) for 48 h, MitoTracker-labeled mitochondria (red), and exosomes colocalized with mitochondria (yellow). Scale bar = 20 μm. (F, G) Immunofluorescence staining and quantification of MMP13, COL2A1, ADAMTS-5, and aggrecan in chondrocytes treated with IL-1β or IL-1β + exosomes. n = 5. *p < 0.05; **p < 0.01.

Article Snippet: Atomic force microscopy (AFM; Multimode 8; Bruker, Germany) was employed for high-resolution hydrogel imaging, and SEM was used to visualize the 3D microstructure of the lyophilized hydrogel.

Techniques: Western Blot, Immunofluorescence, Labeling, Confocal Microscopy, Incubation, Staining

Journal: Theranostics

Article Title: Desktop-stereolithography 3D printing of a radially oriented extracellular matrix/mesenchymal stem cell exosome bioink for osteochondral defect regeneration

doi: 10.7150/thno.31017

Figure Lengend Snippet: 3D printing of radially oriented ECM/GelMA/exosome bioink. (A) Illustration of the SLA printing process. The printing resolution was set to 0.05 mm. (B) CAD file, printing process, and scaffold (stained with methylene blue dye) printed by the 3D printer. The scaffold was observed under microscopy and SEM. (C) The exosome retention ratio of the scaffold for 14 days. (D) In vivo fluorescence imaging of exosomes in PBS and in the 3D printed scaffold. Both groups of exosomes were labeled with Vybrant DiO (green) dye before implantation.

Article Snippet: Atomic force microscopy (AFM; Multimode 8; Bruker, Germany) was employed for high-resolution hydrogel imaging, and SEM was used to visualize the 3D microstructure of the lyophilized hydrogel.

Techniques: Staining, Microscopy, In Vivo, Fluorescence, Imaging, Labeling